1 | Integrated management of blast diseases in rice Jaiganesh, V.
https://doi.org/10.57182/jbiopestic.11.2.82-88 Journal of Biopesticide., 2018; 11(2) : 82-88
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This paper deals about the application of SIR (Systemic Induced Resistance) chemicals and organic sources like Panchakavya to the host along with Bio control agent would enhance disease suppressing mechanisms against rice blast disease. Among the various treatments, seed treatment of Pseudomonas fluorescens (PFS) a native isolation @ 10 g/kg of seeds along Annamalai nagar with foliar application of chemical Nicotinic acid NA1 (15 DAT- days after transplanting @ 100 ppm) and organic product “Panchakavya” (Modified) PK2 (30 DAT @ 5 %) increased plant height, Number of tillers, Number of productive tillers per clump, panicle length, thousand grain weight and minimum disease incidence recorded in IR 50 under pot culture conditions. Percentage of filled grain, grain yield and straw yield are improved when compared to other treatments. Also, Seed and foliar application of bio protectant increased the rhizosphere population.
2 | Identification of bioactive compounds from Mirabilis jalapa L. (Caryophyllales: Nyctaginaceae) extracts as biopesticides and their activity against the immune response of Spodoptera litura F. (Lepidoptera: Noctuidae) Dina Maulina1, 2,*, Sutiman Bambang Sumitro3 , Mohamad Amin 1, Sri Rahayu Lestari 1
https://doi.org/10.57182/jbiopestic.11.2.89-97 Journal of Biopesticide., 2018; 11(2) : 89-97
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Dina Maulina1, 2,*, Sutiman Bambang Sumitro3 , Mohamad Amin 1, Sri Rahayu Lestari 1
3 | Potency of local isolate Paecilomyces from West Sumatera for control of rootknot nematodes (Meloidogyne spp) on vegetables Winarto, Darnetty and Yenny Liswarni https://doi.org/10.57182/jbiopestic.11.2.98-105 Journal of Biopesticide., 2018; 11(2) : 98-105
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Paecilomyces is an antagonistic fungus that can be used as a base material for the manufacture of bionematicides for the control of root knot nematodes (Meloidogyne spp) because it has antagonistic activity as a nematode egg parasite. Utilization of Paecilomyces local isolates has good potentials for the control of parasitic nematodes, especially root knot nematodes. The environmental conditions of fungal isolates affect the ability of fungi in parasitizing nematode eggs. The objective of the study was to obtain local isolate of Paecilomyces fungi which have high potential to control root knot nematodes. Fungal samples were from soil rhizosphere of tomato plants infected by root knot nematodes (Meloidogyne spp) from several locations of vegetable production centers of West Sumatra, namely Alahan Panjang, Agam and Tanah Datar. As results of the research, 10 isolates of Paecilomyces fungi were obtained, 3 isolates from Alahan Panjang (PAP1, PAP2, and PAP3), 3 isolates from Agam (PA1, PA2, and PA3) and 4 isolates from Tanah Datar (PTD1, PTD2. PTD3 and PTD4). All Paecilomyces isolates had potency to control root knot nematode (Meloidogyne spp) with suppression percentage ranged from 55% - 70.9%. The PAP 2 (isolate from Alahan Panjang) had the best ability to control root knot nematodes with suppression percentage of 70.90%.
4 | Efficacy of herbal powders on seed mycoflora and seed quality of oilseeds Maria Sheeba Nazareth, Girish, K. and Syeda Kousar Fathima* https://doi.org/10.57182/jbiopestic.11.2.106-113 Journal of Biopesticide., 2018; 11(2) : 106-113
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The present study involved seed health testing of four oil seeds (mustard, niger, groundnut and sunflower) for seed-borne fungi and seed vigor, and the evaluation of neem and tulsi leaf powders as seed treatment biofungicides. Standard blotter, agar plate and paper towel methods were employed. All the seed samples tested yielded several seed borne fungi. The herbal powders were screened individually and in combination (1:1) at the concentrations of 2, 5 and 10% by seed treatment. The incidence of seed borne fungi from oilseeds was substantially reduced by neem and tulsi powder on seed treatment in comparison to untreated control seeds. The leaf powders also increased seed germination and seedling emergence with increased shoot and root length. These plant powders could possibly be exploited in the management of seed-borne pathogenic fungi to prevent deterioration of oil seeds and to increase their vigor in an eco-friendly way.
5 | Integrated management of leaf miners Liriomyza spp. (Diptera: Agromyzidae) on shallot crops by trap cropping system and arbuscular mycorrhizae Shahabuddin Saleh*, Alam Anshary and Usman Made https://doi.org/10.57182/jbiopestic.11.2.114-120 Journal of Biopesticide., 2018; 11(2) : 11(2): 114-120
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Integrated pest management (IPM) has been recognized as the best solution to minimize the adverse effects of insecticides applied to farmland. This study aims to evaluate the effectiveness of the trapping crop system (TCS) and arbuscular mycorrhizae (AM) against leaf miner, an important horticultural pest throughout the world including on shallot crops in Indonesia. In a factorial research design, cucumbers use in TCS was planted in three different pattern: one rows in the center; TC1, two rows in the middle; TC2, and one-row surroundings the shallot; TC3, while AM consists of two levels that are with (M1) and without AM (M2) application. The variables measured were: population and infestation of leaf miners as well as parasitism at the main and trap crops. The results showed that the leaf miners population was significantly reduced by TCS application but had no relation with AM. The leaf miners population decreased by about 40.1% at the TCS compared with the controls. However, the combination of TCS and AM reduced the leaf miner infestation by about 47.5% where TC3M1 showed the best treatment against leaf miners. Increasing of the parasitoids population and parasitism at the shallot and cucumbers as trap crops as well as the shallot resistance against leaf miner may explain the compatibility of TCS and MA to manage the pest. The study denoted the importance of IPM against shallot leaf miners to support sustainable farming system.
6 | Insecticidal activity of essential oils from six Moroccan plants against insect pests Rhyzopertha dominica, Sitophilus oryzae and Tribolium castaneum Sabrine Idouaarame1, Ouafaa Aniq Filali* 2, Maryama Elfarnini1 and MohamedBlaghen1,3 https://doi.org/10.57182/jbiopestic.11.2.121-127 Journal of Biopesticide., 2018; 11(2) : 121-127
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Six essential oils from Morrocan plants belonging to different botanical families (Lamiaceae, Pinaceae and Verbenaceae), were prepared by hydrodistillation. These essential oils from Morrocan plants, which have a long tradition in adjuvant therapy, were tested for insecticidal activity by the method of microcomputer-atmosphere against three major pests of stored products : Rhyzopertha dominica (F), Sitophilus oryzae (L) and Tribolium castaneum (herbst). The results obtained showed that the essential oils possessed highly significant insecticidal properties against the pests studied. R (+) pulegone, a monoterpene ketone and a major component of the essential oil of Mentha pulegium exhibited the highest insecticidal activity.
7 | Pathogenicity of Helicoverpa armigera Nucleopolyhedro virus (HearNPV) and Lecanicillium sp. against Helicoverpa armigera Sempurna Ginting 1, Teguh Santoso 2, Yayi Munara K.2, Ruly Anwar 2, Lisdar I. Sudirman 3. https://doi.org/10.57182/jbiopestic.11.2.128-135 Journal of Biopesticide., 2018; 11(2) : 128-135
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Helicoverpa armigera (Hubner) is an important pest of corn crops. Lecanicillium sp. and Helicoverpa armigera Nucleopolyhedro virus (HearNPV) are biological control agents that are safe for the environment. This study observed pathogenicity of HearNPV and Lecanicillium sp. against eggs and larvae H. armigera. The results showed that HearNPV application was not able to infect eggs, but the mortality effects of newly hatched larvae and second instars larvae at density of 107 was 100% and 90% respectively, while in mixed applications Lecanicillium sp. and HearNPV on eggs at 107 caused 17.5%, and eventually died were 100%. At 107 concentration with mixure of application (Lecanicillium sp. and HearNPV) mortality was only 26.25%. The value of LT50 in HearNPV application against H. armigera larvae was 2.03 days faster than that of mixed applications on 3.23 days. A conclusion statement HearNPV was more effective against H. armigera than mixed application (HearNPV and Lecanicillium sp.)
8 | Increase in percent purity of β carotene content in organically cultivated tomatoes estimated by SPE and HPLC Nitika Thakur https://doi.org/10.57182/jbiopestic.11.2.136-141 Journal of Biopesticide., 2018; 11(2) : 136-141
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Nutrition and health are the two most important related areas which always need to be refreshed. Tomato has become an important vegetable of the world in view of the increasing demand for the fresh consumption as well as for processing industries. As a fresh commodity and as a processed product, tomato represents a major source of essential nutrients. The present studies were carried out to evaluate the beta-carotene content of organic and conventionally grown tomatoes by combining SPE and HPLC techniques. The analysis of the sample revealed that β-carotene percentage recorded in organic samples were higher than that the control. Sample T₃ organic was recorded with 13.41 per cent purity, followed by T₄ (8.3%). Minimum percentage purity (4.81%) was recorded in the conventional treatment (control). The results clearly indicated a higher percentage of β-carotene in organic treatments, as compared to farmer’s practice (control).
9 | Comparative field efficacy of entomopathogenic fungi and certain new insecticide molecules against leafhoppers, Amrasca devastans (Distant) on Bt cotton MahaLakshmi M.S., Prasada Rao G.M.V. and Prasad N.V.V.S.D. https://doi.org/10.57182/jbiopestic.11.2.142-145 Journal of Biopesticide., 2018; 11(2) : 142-145
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A field experiment was conducted to evaluate the efficacy of certain new molecules as well as entomopathogens as foliar sprays against leafhoppers in Bt cotton at Regional Agricultural Research Station, Lam, Guntur, Andhra Pradesh for two consecutive years. The new molecule, flonicamid 50% WG at two doses i.e. at 75 g a.i./ha and 100g a.i./ha followed by diafenthiuron 50% WP 300 g a.i./ha and buprofezin 25% SC @ 250 g a.i./ha were found promising against leaf hoppers together with high seed cotton yield. New molecules were found significantly superior over to NSKE 5 % and entomopathogens in suppressing the leafhoppers.
10 | The integration efficacy of formulated abamectin, Bacillus thuringiensis and Bacillus subtilis for managing Meloidogyne incognita (Kofoid and White) Chitwood on tomatoes Mohamed S. Khalil1 and Shimaa S. I. Abd El-Naby1
https://doi.org/10.57182/jbiopestic.11.2.146-153 Journal of Biopesticide., 2018; 11(2) : 146-153
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A pot trial was conducted to evaluate the impact of formulated abamectin, Bacillus thuringiensis and Bacillus subtilis alone or in combinations against the root-knot nematode (Meloidogyne incognita) development in tomato plants. Abamectin was the superior treatment which reduced galls and soil populations by 85.87 and 85.13%, respectively. The single treatments showed that the high rate of B. thuringiensis and / or B. subtilis recorded the highest reductions than the low rate. The single treatments of B. thuringiensis and B. subtilis gave reductions in galls ranged from 66.22 to 78.88%, and in soil populations ranged from 70.63 to 80.45%. Moreover, the binary mixtures of abamectin + B. thuringiensis recorded the highest reductions among mixtures in galls (85.20%) and soil populations (76.56%).On the other side, all the applied single treatments increased the tomato plant growth criteria over control. The binary blend of abamectin + B. thuringiensis recorded the highest augmentation in root length, root fresh weight and shoot fresh weight by 41.84, 49.58 and 56.72%, respectively.
11 | Assessing the potential of some Egyptian plants as soil amendments in Meloidogyne incognita management on tomato Hala S. Ibrahim, Mohamed A. Radwan, Abdel Fattah S. A. Saad, Hassan A. Mesbah and Mohamed S. Khalil https://doi.org/10.57182/jbiopestic.11.2.154-160 Journal of Biopesticide., 2018; 11(2) : 154–160
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In a pot experiment, dried plant materials of Brassica oleracea, Bougainvillea spectabilis, Emex spinosa and Erythrina humina were mixed with soil at the rate of 5 and 10 g/kg soil and compared their nematicidal potential with oxamyl, against the root-knot nematode, Meloidogyne incognita infecting tomato plants under greenhouse conditions. In addition, their effects on the growth of tomato plants were also assessed. The results showed that all the amendments exhibited varying degree of reduction compared to control. Moreover, Erythrina humenea recorded the highest reductions in galls/ root system (96.77%), egg masses/ root system (97.99%), eggs/ mass (77.11%) and soil populations/250g soil (93.60%). Except E. spinosa, employing high rate of the tested dried plants gave higher activity in suppressing the nematode parameters than the low rate. Besides highly nemato-toxic potential, B. oleracea, B. spectabilisor, E. spinosa showed phytotoxic effects on tomato when used at the high rate. These dried plant materials at low rate show promising nematicidal activity and may offer possibilities as non-chemical alternatives for the management of M. incognita on tomato.
12 | Molluscicidal toxicity of abamectin against Eobania vermiculata and Theba pisana in vivo and the estimation of GABA-Transaminase activity by HPLC Gaber, M. Abdelgalil1, Hamza, S. Abou-Elnasr2, Mohamed S. Khalil3, Mahmoud S. Abouhamer4, Ahmed M. Osman4 and El-Sayed, A. M. Abdallah5 https://doi.org/10.57182/jbiopestic.11.2.161-168 Journal of Biopesticide., 2018; 11(2) : 161-168
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The molluscicidal toxicity of abamectin against two economic snails namely, the brawn garden snail (Eobania vermiculata) and the white garden snail (Theba pisana), was established under in vivo conditions. Abamectin was used by the topical application technique. The mortality of snails was recorded after 24 and 72 hrs of exposure. The obtained results revealed that E. vermiculata was more resistant to abamectin than T. pisana. The LD50 for E. vermiculata after 24 and 72 hrs of exposure were 0.379 & 0.250 μg/ gm b.w, respectively. Whereas the LD50 for T. pisana were 0.199 and 0.125 μg/ gm b.w after 24 and 72 hrs of exposure, consecutively. On the other hand, the impact of abamectin upon GABA- Transaminase concentration was estimated in both snails. For the first time, the activity of GABA- T was estimated by HPLC. Abamectin has been assessed with 1/2, 1/5 and 1/10 of LD50 for 24 and 72 hrs of exposure. The most effective dose on GABA- T activity was 1/2 of LD50 after 24 and 72hrs of application for both snails.