AUTHOR(S)
Najmun Naher1, Bholanath Mondal2*, Shamim Shamsi3, Md. Abul Bashar3, Md. Rawshan Ali4

ABSTRACT

ABSTRACT

An in vitro experiment was conducted during 2018–2020 atBARI, Bangladesh, Gazipur to screen the antagonistic effects of nine fungaland five bacterial antagonists against Sclerotinia sclerotiorum (Lib.) de Bary.White mold disease of mustard is one of the most important diseases of mustard causedby the phytopathogen S. sclerotiorum. Among the fungal antagonist tested, Aspergillusniger 2, A. niger 3, Penicilium sp, Trichodermaharzianum, T. viride and T.             virens were mosteffective in inhibiting 89.35% to 100% mycelial growth of S.sclerotiorum in the dual culture method on PDA medium.Among these antagonist fungi, Aspergillus niger 2, A. niger 3, T.harzianum, T. viride and T.virens were capable of completely stopping the sclerotia formation of S.sclerotiorum. The Culture filtrates of Trichoderma species viz., T.harzianum, T. viride, T. virens completely(100%) inhibited the radial growth of the test pathogen. The highest inhibition ofradial growth of S. sclerotiorum observed with the volatilemetabolites of Trichoderma viride (90%) followed by T. harzianum (89.39%)and T. virens (89.35%). More than 90% ofmycelial growth was inhibited by Pseudomonas sp. and Bacillussubtilis strain B20 in dual culture on PDA medium and alsoeffective in stopping sclerotia formation. In the volatile method, all thetested antagonistic bacteria showed moderate antagonistic effects against S. sclerotiorum. Further study isrequired to develop effective formulation of those biocontrol agents for controllingthis alarming pathogen in field condition in an eco-friendly manner.

Keywords: White mould, Sclerotinia sclerotiorum, Antagonism, Biological control

DOI
https://doi.org/10.57182/jbiopestic.18.2.156-166

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