ABSTRACT
A total of 178 microbial cultures were isolated fromrhizospheric soil, of which 45 cultures were identified as actinomycetes basedon morphology. After primary and secondary screening, the actinomycete GK-121was found to be a potent biocontrol agent for the rice blast pathogen Pyriculariaoryzae. The culture was identified using conventional microscopy, ScanningElectron Microscope (SEM) and molecular characterization of the 16S rRNAsequence, and showed 99% similarity with Streptomyces maritimus, whichwas further confirmed by phylogenetic analysis. The cultureexhibited a 45 mm zone of inhibition during in vitro screening against P. oryzae. The greenhouse experimentfurther proved the effectiveness of the isolate, as the seeds treated with S.maritimus showed very low disease incidence in plants inoculated with thepathogen P. oryzae, whereas untreated seeds showed high diseaseincidence. This is the first report to suggest thatS. maritimus is effective in controlling the rice blast pathogen, P.oryzae, in vitro and in vivo.
Keywords: Rhizosphere,Actinomycetes, Antiphytopathogenic activity, Molecular characterization,Biocontrol
How to cite this article:
Bahaderjeet Singh, Amritpal Mehta, Vasu Mehta and Mahesh VMahajan. 2025. Isolation and characterization of Streptomyces maritimus exhibitingin-vitro and in-vivo antagonistic activity against rice blastpathogen Pyricularia oryzae. Journal of Biopesticides, 18(2):125-133. DOI:10.57182/jbiopestic.18.1.125-133
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