Bacillusthuringiensis (Bt)was investigated in three different habitats (vegetable and crops-cultivated soils,phylloplanes and insect guts) of Bangladesh. A total of 61 Bacillus cereus-likeisolates were obtained by selective methods and 57 of those were identified as Bacillusthuringiensis isolates based on their hemolytic activity, presence ofparasporal crystal proteins, plasmid profile and crystal protein profile. Theprevalence of Bt was highest (60%) in soil samples followed by leaf andinsects. Five different types of parasporal crystal proteins (spherical,bipyramidal, irregular pointed, cuboidal and irregular shaped) were observedamong the isolates which indicates the diversity of the local Bt isolatesexamined. In addition, confirmation of the strain identification was done using16S rDNA gene sequencing. Plasmid analysis recovered from the isolatesyielded at least one 15kb DNA, which is well comparable to the referencestrain, B. thuriginesis kurstaki var. HD-73. Further similaritybetween the test and standard strains was observed while analyzing crystalproteins on a SDS-polyacrylamide gel that produced major bands of Cry1, Cry2and Cry9 type proteins. Bioassay performed with isolates Bt-01i, Bt-25f andreference strain, Bt kurstaki var. HD-73 against pulse beetles Callosobrochuschinensis resulted LC50 values of 0.30, 0.72 and 0.22 mg/ml crude proteinsrespectively which demonstrated their competency in Biopesticide production andapplication.
How to cite this article:
AsaduzzamanShishir, Asma Akter, Md. Hasibul Hassan, Golam Kibria, Mohammad Ilias, ShakilaNargis Khan and Md. Mozammel Hoq*. 2012. Characterization of locally isolated Bacillus thuringiensis for thedevelopment of Eco-friendly biopesticides in Bangladesh. Journal ofBiopesticides, 15(0):216-222.
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