The efficacy ofleaf extracts of Momordica charantia and Bacillus thuringiensis hasbeen proven against larvicidal and pupicidal activities of the malarial vector,Anopheles stephensi. The present study investigated the larvicidal andpupicidal activity against the first to fourth instar lavae and pupae of thelaboratory-reared mosquitoes, An. stephensi. The plant extract showedlarvicidal and pupicidal effects after 24 h of exposure. All larval instars andpupae have considerably moderate mortality; however, the highest larval andpupal mortality was the methanol extract of leaf M. charantia againstthe first- to fourth instars larvae and pupae values of LC50=I instar was 93.45ppm, II instar was 123.74 ppm, III instar was 167.17 ppm, and IV instar was216.15 ppm, and pupae was 256.66 ppm, respectively and bacterial insecticide, B.thuringiensis for the first-to fourth instars larvae and pupae recorded theLC50 values: 53.47 ppm, 62.09 ppm, 79.15 ppm, 95.39 ppm, and 105.76 ppm for theI- IV instar larvae and pupae, respectively. The combined treatment recordedthe values of LC50 85.09 ppm, 90.51 ppm, 111.91 ppm, and 137.61 ppm for I to IVinstars and 154.40 ppm for pupae respectively. The results of the presentinvestigation revealed effect of methanolic extract of M. charantia and B.thuringiensis for controlling of larvicidal and pupicidal properties ofagainst malarial vector, A. stephensi.
How to cite this article:
JayapalSubramaniam*, Kadarkarai Murugan and Kalimuthu Kovendan. 2012. Larvicidal and pupcidal efficacy of Momordica charantia leaf extract andbacterial insecticide, Bacillusthuringiensis against malarial vector, Anophelesstephensi Liston. (Diptera: Culicidae). Journal of Biopesticides, 15(0):163-169.
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