Dynamics of Trichoderma spp., is considered as an ecofriendly process due to their prolific effect of secondary metabolite when used as biological control agent in agriculture for crop protection. Fusarium wilt is one of the most serious disease of crop which crop loss in short time due to vascular wilt with the support of secretary protein and enzymes i.e. SIX, Sg1, TOM, pel D. Here, we have selected two pathogens one causing the Tomato wilt and is the other Chickpea wilts for in vitro analysis. Moreover, we had been used of analysis approach as in vitro screening by dual technique and in silico by molecular docking based on AutoDock vina 4.0.In-vitro screening, the result was better with T2 (Trichoderma virens) in comparisontoT1, T5, T7, T12, T13,T14, Th, Tv and control in case of both pathogens. In silico screening, (101849747) ligand was found to the best when compared to 379 compound with SIX, Sg1, TOM, pelD based on binding energy as -15.5, -19.6,-19.7,-16.0.