AUTHOR(S)
Alina Paul, and Jayaraj, R.
ABSTRACT
The efficacies of biopesticides are influenced by various storage conditions including
environmental factors and stability is one of the key concerns. Emulsifiable concentrates (ECs)
prepared from the bark crude extract of Pachygone laurifolia plant and its active fraction showed
significant feeding deterrence on Spodoptera litura, a poly-phytophagous insect pest attacks many
vegetables and economically important cultivators. Effect of various factors including storage
duration, storage temperature, exposure to sunlight and ultraviolet light on the stability of the
formulations prepared from two different concentrations (1 and 2.5%) were evaluated. The EC
preparations of both crude extract and active fraction showed significantly high anti-feedant
activity. In case of EC from crude extract, the accelerated storage conditions shown to decrease the
anti-feedant activity at the lower concentration of 1% however no significant change was noted at
2.5% concentration except at 200C. The EC preparation from active fraction, up on long term
storage at 30 ± 20C for different time intervals, significant loss of activity was noted at 12-month
storage in both concentrations of crude extract EC and active fraction EC preparations. Exposure
to direct sunlight has significantly reduced the activity in a time dependent manner. UV light
exposure has significantly reduced the activity in low concentration (1%) EC formulation of both
crude extract and active fraction however, the higher concentration preparations were stable up to
8 hrs. The study showed that, EC formulations of P. laurifolia crude extract and active fractions
were sable at higher concentration and can be used for field applications.
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Keywords: Biopesticide formulation, Shelf life, Thermal stability, Photodegradation, UV light
degradation.
DOI
https://doi.org/10.57182/jbiopestic.17.1.30-41
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How to cite this article:
Alina Paul, and Jayaraj, R. 2024. Influence of environmental factors on stability and antifeedant
activity of biopesticide formulations of Pachygone laurifolia (DC.) L.Lian & Wei Wang
(Cocculus laurifolius) against Spodoptera litura (Lepidoptera : Noctuidae). Journal of Biopesticides, 17(1):
30-41.