Journal of Biopesticide

Journal of Biopesticide

2023, Vol. 16, Issue2

INDUCING THE RESISTANCE AGAINST TOBACCO MOSAIC VIRUS IN PEPPER USING THE EXTRACTS OF DATURA STRAMONIUM AND GANODERMA LUCIDUM


AUTHOR(S)
1Raghad Naif Mheedi and 2Hameed Hamoud Ali

ABSTRACT

Pepper plants treated with the same Tobacco mosaic virus (TMV) showed no signs and symptoms of disease, in contrast to plants treated with these compounds as a control, which was thought to act as an inhibitory start to the viral infection. This resistance was sparked by the pepper plants' exposure to the TMV virus. Furthermore, the ELISA test findings indicated a wave reaction based on the yellow color that developed in the ELISA plate wells containing the extracts of pepper plants that have been treated with Ganoderma lucidum extract. When compared to the control treatment, which is infected with the virus alone, the wave absorption amount at 405 nm was 0.296 nm, indicating that the highest percentage of virus duplication occurred (69%) after the second week of the G. lucidum treatment and before six days of infection. On the other hand, 58% of the virus was duplicated when the G. lucidum extract was sprayed three days prior to the virus infection, and the wave absorption value was 0.404 nm. The values of wave absorption following splashing with the same extract were 0.612 and 0.598 after three and six days of virus infection, respectively, and the percentages of virus duplication were 37 and 38%. Lastly, it should be emphasized that, rather than using chemical pesticides that are unsafe to use against the causes of plant diseases, more research must be done in the future regarding the extraction of these anti-viral plant extracts as well as extracts of naturally occurring substances that are environmentally friendly.
Keywords: Induced antiviral proteins (IAVPs), Tobacco mosaic virus, Datura stramonium, Ganoderma lucidum.


DOI
https://doi.org/10.57182/jbiopestic.16.2.179-184

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How to cite this article:

Citation: Raghad Naif Mheedi and Hameed Hamoud Ali. 2023. Inducing the resistance against Tobacco mosaic virus in pepper using the extracts of Datura stramonium and Ganoderma lucidum. Journal of Biopesticides, 16(2):179-184. DOI:10.57182/jbiopestic.16.2.179-184

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