Echinochloa crusgalli (Barnyard grass) is one of the most invasive paddy (Oryza sativa L.) weed. Pure culture isolates of several fungi including Fusarium oxysporum (SBT # 17) and Curvularia lunata (SBT # 30) obtained from field sites were tested as candidates for the microbial control of barnyard grass. A survey was conducted in the paddy fields of eight southern districts of Andhra Pradesh to document the fungal pathogens of barnyard grass. Pure cultures were isolated from the naturally-infected barnyard grass leaves collected from different paddy - growing areas. A total of 51 fungi were isolated and two isolates were pathogenic on E.crusgalli under laboratory condition (> 70%). Furthermore F.oxysporum (SBT # 17) and C. lunata (SBT # 30) exhibited more pathogenicity under green house conditions on E. crusgalli and holds a great promise as biocontrol agents of barnyard grass.