AUTHOR(S)
Manoja Das, Sumitra Mandal, Rajsekher Padhy and Pragyna P Tripathy

ABSTRACT

Green gram (Vignaradiata L.) is a popular edible legume of India. The plant is cultivated by the local farmer as cash crops. Rhizobium sp. was isolated from the root nodules of green gram. The pure culture of the isolated bacterium was carried out at the laboratory with Yeast- Extract Mannitol (YEM) Agar medium. The characterization of the bacterium was made by morphological and biochemical tests. Production of siderophores by the Rhizobium bacterium was qualitatively measured by Chrome Azurol S (CAS) agar plate method. Antagonistic behaviour of the Rhizobium  isolates against two phytopathogenic microorganisms such as Rhizoctonia solani and Rhizoctonia oryzae were studied by the agar co-cultivation method. The zone of inhibition was recorded for both the pathogens are 46% and 51% respectively. The results indicated that Rhizobium species are capable to resist the growth of both the studied phytopathogens at in vitro condition. Therefore, Rhizobium sp. is a good candidate to control the growth of both the studied phytopathogenic microorganisms and can be used as a biocontrol agent for the same.

Keywords: Biocontrol; Rhizobium species; phytopathogens; legume plant

DOI
https://doi.org/10.57182/jbiopestic.14.1.27-31

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