Lactone glycoside, the active ingredient present in Cleistanthus collinus (Roxb.) Benth. possessing variety of anti insect properties like antifeedant, insecticidal and insect growth regulatory activity was bioasayed on third, fourth and fifth instar Spodoptera litura Fab. larvae to determine its median effective concentrations. Median deterrency index (DI50), median lethal concentration (LC50) and median emergence inhibition (EI50) were worked out for antifeedant, insecticidal and insect growth regulatory activity respectively through regression and probit analysis. The anti insect activity was dose dependant. The activity varied with increase in concentration in the following order viz., insect growth regulatory, insecticidal and antifeedant action while Median emergence inhibition was at 0.17 ppm, 0.26 ppm and 0.38 ppm, median lethal concentration and median deterrency index were at 1.02 ppm, 1.71 ppm, 2.045 ppm and 60.66 ppm, 68.47 ppm, 71.10 ppm for third, fourth and fifth instars respectively. Further, the activity was also found to be age dependant. As the larval age increased, the required median effective dose also increased. Multi pronged anti insect activity of the lactone glycoside was compared with other botanicals and discussed in detail.