Journal of Biopesticide

Journal of Biopesticide

2010, Vol. 3, Issue1

DISRUPTION OF OOCYTE DEVELOPMENT AND VITELLOGENESIS IN ORYCTES RHINOCEROS TREATED WITH METHANOLIC EXTRACT OF EUPATORIUM ODORATUM LEAVES


AUTHOR(S)
K. B. Sreelatha and P. R.Geetha

ABSTRACT

Effect of methanolic extracts of Eupatorium odoratum (Compositae) leaves on the ovary of the coconut pest, Oryctes rhinoceros L.(Coleoptera: Scarabaeidae) was studied. Topical application of 10 and 20 µl extracts resulted in morphological, morphometric, gravimetric and histological aberrations in the ovary of the beetles. Ovariole of O. rhinoceros was telotrophic meroistic and consists of terminal filament, germarium and vitellarium. Terminal filaments of the ovarioles of the experiment are short and are not interconnected whereas in the control they are long and interconnected. Interfollicular tissue was distinct in the control and appears indistinct in the treated. Vitellarium of the treated insects shows fusion and abnormal orientation of follicles. Number of follicles and weight of ovary were reduced significantly (p < 0.05). Length of germarium was reduced in10µl treatment while it was increased in the 20 µl. Vitellarium and ovariole length was increased and reduced in 10 µl and 20 µl treated insects respectively. Topical application of the extract resulted in histological abnormalities including vacuolation of germarium, chromatin condensation and defective development of the follicular epithelium, lateral trophic, interfollicular and prefollicular tissues, vacuolation of ooplasm and abnormal /arrested vitellogenesis and choriogenesis. Vitellogenesis in O.rhinoceros can be disrupted by the application of E. odoratum extracts and this plant can be considered as a potential candidate for regulating this pest.


DOI
https://doi.org/10.57182/jbiopestic.3.1.253 - 258

PAGES: 253 - 258 | 0 VIEWS | 0 DOWNLOADS

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