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The side-effect of Azadirachta indica (A. Juss) leaf extract on certain biochemical parameters of a freshwater fish, Labeo rohita was studied for a period of 25 days at sublethal concentration. The median lethal concentration (1.035 g/L) of A. indica for 24 h was calculated and 1/10th of the toxicant (0.1035 g/L) was taken for sub-lethal study. During the exposure period the glycogen content in the liver and muscle of leaf extract treated fish increased up to the 15th day and then declined, whereas the protein level decreased in the liver and muscles throughout the study period. The alterations of these biochemical parameters can be effectively used as nonspecific biomarkers against plant extract toxicity stress and also help safer usage of plant extracts in aquaculture farms.
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