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Drosophila is a simple and genetically tractable system, it has been used to unravel and understand different aspects of biology, including neurobiological studies. Nicotine is a generally occurring liquid alkaloid. This botanical is known as contact poison and it generally affects the central nervous system. It has been used as an insecticide, it stimulates nicotinic receptors and neurosecretion at the neuromuscular junction. For the present study 0.05% crude extract of tobacco (nicotine) was used to study the effects on various stages and behaviour such as, larval pupation site preference (PSP), locomotory behaviour, mating success, fecundity and productivity in Drosophila melanogaster. Synchronised eggs were collected and allowed to hatch, from these standard cultures first instar larvae were transferred to glass vials containing nicotine mixed media. Yeast dissolved in nicotine was added every day to larvae for feeding. Fifty first instar nicotine treated larvae were allowed till pupation in vials to study PSP, after pupation the pupa were counted and analyzed. Nicotine treated third instar larvae were used for analyzing larval locomotory behaviour. Larval patterns were taken on transparent paper placed on agar coated petri dish for five minutes and larval path lengths were measured. About thirty pairs of five day old adult flies were collected for mating success study. Mated females were transferred to separate vials to count eggs layed every day and pupa after pupation to analyze fecundity and productivity. Treated larva showed a significant difference than control in larval pupation site preference, locomotory behaviour, mating success, fecundity and productivity.
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