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Various bioassay methods were employed to screen SlNPV against S. litura on different host plants. In the first experiment S. litura larvae were reared on different host plants and when they completed second instar, they were transferred to semi-synthetic diet which was treated with different doses of SlNPV. The LC50 value against the pest which was reared on different host plants were as follows: cabbage 0.42, cotton 0.61, potato 0.75, groundnut 0.93 and rose 1.28 POB/mm2. In the second experiment, larvae of S. litura were reared on semisynthetic diet up to second instar and subsequently they were fed on different host plants, which were treated with SlNPV. The LC50 value was highest in rose (1.81) followed by groundnut, potato, cotton and cabbage (0.40 POB/mm2). In the third experiment, larvae of S. litura were reared on different host plants till they complete second instar and they were screened against SlNPV in the laboratory using leaf disc method. The LC50 value was highest in rose followed by groundnut, potato, cotton and cabbage (1.02 POB/mm2). In the above three experiments, the LC50 value was lowest in cabbage and was highest in rose.
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