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Trichoderma viridi is a major biocontrol agent against a wide range of phytopathogenic organism in economic important crops and extensively used in various parts of the world and known to degrade complex organic molecules into simpler solubilize forms which helps to improve soil fertility. Humic acid is an organic fertilizer which enhances the plant growth. The aim of the present study is to solubilize lignite to humic acid by T.viridi under defined conditions. The solubilization efficacy of T.viridi was carried out in Czapex Dox broth containing different concentration of lignite such as 1, 1.5, 2, 2.5 and 3% inoculated with 0.6 ml spore suspension under shaking condition at 30°C for 7 days. The humic acid was obtained at 2.5% concentration and comparatively very less amount of humic acid in other respective concentrations. The media was optimized for evaluation of enhanced solubilization by Placket Burman Model reveals 0.2% of humic acid at 2.5% of lignite supplemented media. The media optimization was especially concerned with addition of various nutrient sources such as Glucose and Yeast extract. Moreover plant growth promoting efficacy of humic acid derived from lignite solubilization with T.viridi was done is sorghum plants grown in pots reveals that T.viridi solubilized humic acid recorded maximum plant growth than synthetic humic acid and untreated control. From this study it is clear that biosolubilization of humic acid enhances plant growth and biocontrol efficacy against phytopathogenic organism.
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