Journal of Biopesticide

Journal of Biopesticide

2010, Vol. 3, Issue1

LABORATORY EVALUATION ON THE POTENTIAL OF ENTOMOPATHOGENIC FUNGI, NOMURAEA RILEYI AGAINST TOBACCO CATERPILLAR, SPODOPTERA LITURA FABRICIUS (NOCTUIDAE: LEPIDOPTERA) AND ITS SAFETY TO TRICHOGRAMMA SP.


AUTHOR(S)
S. P. Shanthakumar, P. D. Murali, S. Malarvannan, V. R. Prabavathy and Sudha Nair

ABSTRACT

Tobacco caterpillar, Spodoptera litura was reared in semi-synthetic diet and the different stages were maintained for bioassay. Entomopathogenic fungi, Nomuraea rileyi was sub-cultured using Potato Dextrose Agar (PDA). Spore suspensions of four different concentrations (2.4 × 107, 2.4 × 106 and 2.4 × 105, 2.4 × 104 conidia/ml) were prepared from the 15 day old culture of the fungi for evaluation. A preliminary study on N. rileyi against S. litura larvae was done. Cypermethrin 25 EC, Neem Plus and untreated were used as controls. Though the pupation was not suppressed as expected, the pupal weight, length and duration were severely affected. The malformed adults were the highest (96.7%) in pupae treated with 2.4 × 106 spore concentration. Subsequently, the fecundity was completely arrested in N. rileyi at 2.4 × 107, 2.4 × 106 and 2.4 × 105 spore conc. The biosafety of N. rileyi against Trichogramma japonicum and Trichogramma chilonis was confirmed by monitoring the parasitization and male-female ratio and proved that the N. rileyi was safe to these egg parasitoids.


DOI
https://doi.org/10.57182/jbiopestic.3.1.132-137

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