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Tobacco caterpillar, Spodoptera litura was reared in semi-synthetic diet and the different stages were maintained to conduct bioassay. Entomopathogenic fungi Beauveria bassiana was sub-cultured on Potato Dextrose Agar (PDA). Spore suspensions of four different concentrations (2.4 × 107, 2.4 × 106, 2.4 × 105, 2.4 × 104 conidia/ml) were prepared from the 15 day old culture of the fungi. A preliminary study on B. bassiana against S. litura larvae was done. Cypermethrin, neem and untreated (sterile water) were used as controls. The least pupation (43.33%) was observed in larvae treated with the highest spore concentration (2.4 × 107) of B. bassiana. A sequential follow up from this assay was done on the resulting pupae and adults, if any. Further treatment of the resultant pupae caused mortality and adult malformation. The healthy moth emergence was least in (2.4 × 104) spore concentration of the treatment, while the fecundity was completely arrested in the highest concentration.
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