Biological control of plant pathogens using antagonistic fungi can be successfully utilized especially within the frame work of integrated disease management system. Efficacy of different isolates of Trichoderma species against two pathogenic fungi causing leaf spot (Alternaria spinaciae) and wilt (Fusarium oxysporum f. sp. spinaciae) on spinach (Spinacea oleracea L.) by dual culture technique

under in vitro conditions. Generally Trichoderma used as a biological control for the inhibition of growth of the pathogens. Different isolates i.e. Trichoderma viride (12), T. harzianum (12), T. koningii (07), T. pseudokoningii (05) and T. virens (10) strains were isolated and used for antagonistic study. Results indicated that, among T. viride isolates, Tv1 and Tv5 showed maximum antagonism, but in case of Tv7 and Tv10 showed minimum antagonism against A. spinaciae. In case of F. oxysporum f. sp. spinaciae showed significant inhibition by Tv4 and reduced the percent inhibition by Tv8 and Tv6. In case of T. harzianum isolates, Th3 and Th10 showed maximum

inhibition on A. spinaciae while in F. oxysporum f. sp. spinaciae, Th5 increased the inhibition and decreased in Th3 and Th7. Trichoderma koningii showed better results in Tk7 isolate whereas decreased Tk4 over A. spinaciae, but in F. oxysporum f. sp. spinaciae reduced rate was more in Tk4 and Tk2. Isolates of T. pseudokonongii found maximum inhibition in Tp5 over A. spinaciae and

significant inhibition in Tp3 over F. oxysporum f. sp. spinaciae. Trichoderma virens isolates were found significant inhibition in Tvr4 and Tvr9 and 50% reduction over A. spinaciae and Tvr4 increased the percent inhibition and decreased the Tvr7 in case of F. oxysporum f. sp. spinaciae.