An important aspect of an NPV biocide is its safety to different non-target organisms. The safety of the formulated and crude HpNPV against second instar silkworm Bombyx mori larvae was tested. Larvae fed with crude virus and formulated HpNPV did not show variation in larval weight, pupation percentage, fresh weight of cocoons, shell weight and fecundity. Bird fed with NPV infected H. puera larvae, presence of the viral occlusion bodied was noted in the faecal droppings within an hour of post feeding. Presence of viral particles in the faeces was observed until 24 hrs postfeeding. This suggested that the viral particle did not remain in the body of the bird for long . The test bird behaved normally and remained healthy throughout the observation period of three months suggesting no deleterious effect of HpNPV. Formulated and semi-purified HpNPV was also tested against three cell lines such as human larynx cell line (Hep2), Green monkey kidney cell line (Vero) and Spodoptera frugiperda ovary cell line (Sf-9). After inoculation with the virus, the cell lines did not show any symptom of infection. These studies have explicitly proved the safety of HpNPV.