Biofoliar spray is a technique of feeding plants by applying nutrients directly on their leaves in a ready form. The objective of the current study was to investigate the interactive biofoliar formulation on their concentrations (15ppm, 30ppm and 45ppm) to enhance plant nutrients (N, P, K, OC, S, Ca, Mg, Zn, Fe, Mn, and Cu) of different mulberry varieties (S-1, S-146, S-1635, BR-2 and AR-14) without harming mulberry ecosystem. Mulberry leaves were collected after biofoliar application and analyzed by using Flame Photometer and Atomic Absorption Spectrophotometer and increase sign in the nutrient concentration of selected mulberry varieties.  The investigation implies that, among five mulberry varieties, BR-2 with 45 ppm concentration performed well and enhanced plant nutrients significantly followed by S-146 and AR-14.