Journal of Biopesticide

Journal of Biopesticide

2013, Vol. 6, Issue2

PRELIMINARY CHEMICAL ANALYSIS OF EXTRACTS FROM ENCOSTERNUM DELEGORGUEI USING GAS CHROMATOGRAPHY MASS SPECTROSCOPY


AUTHOR(S)
C. J. Zvidzai 1* , C. Chidewe 2 and R. Musundire1

ABSTRACT

The methanol extract of Encosternum delegorguei was quantitively analyzed through GCMS which had plasma-ionization detector (PID), quartz capillary column ZВ-5 (polydimethylsyloxane, 5 % of phenyl groups), identified and produced the following 18 compounds which were C2-C13 aliphatic hydrocarbons, aldehydes, furanones, aromatic, oxo-alkenals, esters, ketones, lactones and ethers. The
compounds were identified as 2-hexenal, 1-methylbutyl formate, 3-methyl-3-heptanol, octenal, 2,4- dimethyl-3-heptanol, 1,1-diethoxy butane, tridecane, 6-trideceny-4-ne, 4,6-nonadien-8-yn-3-ol, 4,5- dimethyl-1,3-dioxolane-2-cyclohecyl, 3-heptanol, 2-hexenal diethyl acetal, 3-ethoxy pentane, 1,1- dimethoxy heptanes, 2-heptanol acetate, 2-methyl-4-pentanal, 5-ethyl-2-furanone and 2-butoxy pentane. FTIR spectra of the chitin sample from E. delegorguei indicated their good defatting with alcohol and deacetylation process. According to the spectra obtained, yield of chitin is low because of poor acetone treatment. The characteristic absorption bands at 1560, 1655 cm-1 and in the vicinity of 3265 and 3100 cm-1 corresponding to the stretching vibration C=O and NH (-NHCOCH3) were of low intensity, and C-H stretching vibrations at 2926 and 2864 cm-1 showed a high intensity. The elemental analysis produced the following percentages of chitin elemental constituents of carbon 46.11 + 0.055; hydrogen 6.63 + 0.002; nitrogen 7.06 + 0.077; with a C/N = (46.11/12 )/(7.06/14) =7.62. Currently we are determining the chemical compounds using standards to elucidate, evaluate the qualitative constituents and in near future the plant can be utilized for pest management. 


DOI
https://doi.org/10.57182/jbiopestic.6.2.211-218

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