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RH-2485 is a molt accelerating compound and provides effective control of a wide range of key lepidopteran pests. It acts as an ecdysone agonist. The chemical upon absorption into the haemolymph of the insect, binds to the ecdysone receptor which initiates the moulting process. As the normal process disrupted the insects are prevented from shedding its old cuticle. The larvae die of dehydration and starvation within 2-5 days. Hence the toxicity tests were conducted for Helicoverpa armigera (Hub.) to fix the LD50.The LD50 of molt accelerating hormone RH-2485 was estimated by Bouquet method. It was found that the LD50 was 7.68, 15.95, 92.93 and 142.16 ppm for I, II, III and IV instar larvae of H. armigera respectively. It was concluded that the earlier instar was found to be more susceptible with lesser LD50 values and as the instars advances the values of LD50 was found to be increasing. Hence this formulation was found to be more toxic for the different instars of H. armigera and can be included in the Integrated Pest Management systems.
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