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Investigations were carried out to assess the efficacy of crude protein of some of Bacillus thuringiensis isolates against lepidopteran pest viz., cabbage leaf webber (CLW) Crocidolmia binotalis Zel. mortality. The native isolate 1526B/b recorded highly virulent (LC50 = 682.420 ppm) where as M-126 recorded lowest virulent (LC50 = 6572.822 ppm). To identify the spectrum of insecticidal genes in the native B. thuringiensis isolates, the polymerase chain reaction (PCR) was carried out and the results show that none of the isolates amplified cry3, 4 and 18 genes.
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