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Beneficial soil bacteria contributes a good share in maintaining the fertility of agronomical lands naturally .This work was undertaken with the aim to isolate, identify, characterize and study growth pattern of these non targeted beneficial soil bacteria under the influence of organophosphates and Biopesticides. In this regard the morphological examination followed by biochemical characterization of isolated bacterial culture was done .Further species level identification was carried out based on 16S rDNA sequence method. The results of BLAST and phylogenetic analyses revealed 1318nt and 1347nt contig region of sample F and H which were characterized as Bacillus cereus strain JY9 and Methylobacterium sp. HJM27 respectively, found homologous with sequence with Genbank accession no. HQ833026.1 and HM243761.1. Growth of Bacillus cereus was most adversely affected by Dimethoate followed by Phorate. The effect of Malathion and Chloripyrifos were not significant on its growth. Similarly Methylobacterium sp under the influence of Dimethoate, Chloripyriphos and Phorate showed an increased generation time whereas in presence of Malathion, it showed a normal growth curve similar to that of control. The growth pattern of both the species were not much affected by biopesticides. Our results emphasizes on less use of organophosphates in agroecosystem to reduce biotic stress on normal beneficial soil bacteria, that would ultimately be helpful in better crop yield, thereby maintaining natural ecological balance.
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