An investigation on the toxicity of two biopesticidal plants – Carica papaya and Nerium oleander aqueous leaf extracts to Oreochromis mossambicus. The LC50 value of Carica papaya and Neriumoleander aqueous leaf extracts was 700 ppm and 400 ppm respectively. The sub lethal concentration for C. papaya and N. oleander was 70ppm and 40ppm respectively. The fishes wereexposed to the sub lethal concentration for a period of 14 days. At the end of 7th and 14th day thefishes were dissected and the gill, liver and intestine were removed from exposed and control groupfishes. The tissues were processed and sectioned at 4µm and then were stained with Haematoxylin- Eosin. The observation of the slides was done under light microscope at 40x magnification andphotographed. The exposed group showed histopathological changes in the tissues, such as: shrunken and narrow secondary gill lamellae, mild to moderate infiltration of inflammatory cells inthe primary and secondary gill lamellae, vacuolar degeneration of epithelial cells of intestinal villi, massive infiltration of inflammatory cells throughout the base of the villi and disruption of epithelial cells. The liver showed swollen nucleus, hydrated and vacuolar degeneration of hepatocytes and mild pockets of infiltration of inflammatory cells.
How to cite this article:
Shoeiba Tasneem, Syeda Hina Kauser and Rafath Yasmeen. 2014.Toxicity of two biopesticidal plants aqueous leaf extracts to Oreochromis mossambicus –histopathology of gill, liver and intestine. Journal of Biopesticides, 7(2):124-131.
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