The present investigation was undertaken to isolate different strains of Pseudomonas fluorescens from various agroecological zones or crop’s rhizosphere like moong, brinjal, rice, chilli, mustard, chirchida and tomato. Totally eight micro flora resembling Pseudomonas fluorescens were isolatedand three isolates were confirmed as P. fluorescens (strain P.f.01, strain P.f.05 and strain P.f.07). Pseudomonas fluorescens strains P.f 07 were found most effective with the highest antagonisticactivity against three fungal pathogen and show maximum inhibition of mycelial growth of Fusariummoniliforme (65.45%), Rhizoctonia solani (68.23%), and Alternaria alternat(48.13%).