Journal of Biopesticide

Journal of Biopesticide

2011, Vol. 4, Issue2

PATHOGENICITY AND HAEMOLYMPH PROTEIN CHANGES IN EDESSA MEDITABUNDA F. (HEMIPTERA:PENTATOMIDAE) INFECTED BY PAECILOMYCES LILACINUS


AUTHOR(S)
Shamela Rambadan, Hamraji Jugmohan and Ayub Khan1

ABSTRACT

Adult Edessa meditabunda were found to be naturally infected by the entomopathogenic fungus Paecilomyces lilacinus (ARSEF 10225) for the first time in Trinidad in the field and consequently laboratory experiments were conducted to determine its efficacy. Mean in vitro radial colony growth for P. lilacinus was the fastest at 200C and slowest at 350C. The LC50 for adult E. meditabunda using P. lilacinus was determined as 6.32 x 106 spores/ml. At this concentration, 50% mortality (ie.LT50) of E. meditabunda was achieved in 1.95 days. Haemolymph of adult E. meditabunda was extracted and used in the identification of proteins by polyacrylamide gel electrophoresis (SDS-PAGE) and the total protein content by the Lowry’s method. There were a total of four protein bands present in the haemolymph of control adults, all of which had molecular weights more than 138.7 kDa. There was gradual increase in the number of bands from day 1-3 post infection by P. lilacinus. Two new protein bands (104.7 kDa and 49.5 kDa) appeared on Day 3 post infection. The total haemolymph protein concentration was significantly higher in the control compared to all three days post infection.


DOI
https://doi.org/10.57182/jbiopestic.4.2.169-175

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