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Bio assays were conducted with MUCL 38502 strain of Beauveria basiana (Balsamo) Vuillemin and MUCL 8237 of Metarhizium anisopliae (Metschnikoff) Sorokin cultured on SDA and SDA supplemented with host insect larval extract against third instar larvae of Spodoptera litura Fab. The trials were conducted at the Department of Entomology, College of Agriculture, GBPUA&T, Pantnagar to determine the effect on biological properties and virulence of the fungi. Each fungus responded differently to the supplementation. However, in both the fungi the bio mass, linear growth, conidial count and viability of the conidia were increased with the supplementation of larval extract. The LC50 and LT50 values of MUCL 38502 grown on SDA medium and medium supplemented with larval extract were 14.85 and 9.65 X105 conidia ml-1 and 123.02 and 113.95 h at 5X 107 conidia ml-1 respectively. The LC50 and LT50 values recorded with MUCL 8237 were 45.23 and 21.32 X105 conidia ml-1 and 138.72 and 130.93 h at 5X107 conidia ml–1. The virulence of both the fungal isolates increased when grown on SDA medium supplemented with larval extract.
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